Fig. 2: Rap1 activates NF-κB transcriptional activity. | Cell Death & Disease

Fig. 2: Rap1 activates NF-κB transcriptional activity.

From: Rap1 deficiency-provoked paracrine dysfunction impairs immunosuppressive potency of mesenchymal stem cells in allograft rejection of heart transplantation

Fig. 2: Rap1 activates NF-κB transcriptional activity.

a Rap1−/−-MSCs were transfected with pNF-κB luciferase reporter pNFκB-Luc. b Transfection of an empty pNF-κB-Luc was set as controls. The amounts of expression plasmid for Rap1 (a, b) and IκB DN (c) were progressively increased as indicated from 1 to 5 µL (60 ng of DNA/µL). RLA was expressed as firefly luciferase activity after it was normalized to Renilla luciferase activity (n = 3 for each group). *p < 0.05; N: non-significance, IκB DN: IκB-dominant negative, RLA: relative luciferase activity.

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