Fig. 2: SLIT2 and ROBO1 promote proliferation and inhibits apoptosis of OS cells in vitro. | Cell Death & Disease

Fig. 2: SLIT2 and ROBO1 promote proliferation and inhibits apoptosis of OS cells in vitro.

From: SLIT2/ROBO1 axis contributes to the Warburg effect in osteosarcoma through activation of SRC/ERK/c-MYC/PFKFB2 pathway

Fig. 2: SLIT2 and ROBO1 promote proliferation and inhibits apoptosis of OS cells in vitro.

a, b Knockdown of ROBO1 or SLIT2 inhibited U-2OS and Saos-2 cells proliferation using the cell counting kit (CCK)-8 assay, Values are means ± SD, **p < 0.01, ***p < 0.001. c Silencing of ROBO1 or SLIT2 suppressed OS cells (U-2OS and Saos-2) proliferation using colony formation assay. Values are means ± SD, **p < 0.01, ***p < 0.001. d Representative images of BrdU incorporation in U-2OS and Saos-2 cells transfected with ROBO1 or SLIT2 shRNA and negative control shRNA. BrdU is shown by red fluorescence, and the cell nuclei were stained with DAPI (blue fluorescence), Scale bars = 100 μm. e Cell cycle was determined in U-2OS and Saos-2 cells by flow cytometry after transfected with the ROBO1 sh-RNAs. The diagrams quantified cell fractions in the G1, S, and G2/M fractions were shown, *p < 0.05, **p < 0.01, ***p < 0.001. f, g Knockdown of ROBO1 or SLIT2 significantly induces U-2OS and Saos-2 cell apoptosis, *p < 0.05, **p < 0.01, ***p < 0.001.

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