Fig. 4: bFGF-induced cell migration is mediated through the RSK2 signaling pathway. | Cell Death & Disease

Fig. 4: bFGF-induced cell migration is mediated through the RSK2 signaling pathway.

From: Kaempferol targeting on the fibroblast growth factor receptor 3-ribosomal S6 kinase 2 signaling axis prevents the development of rheumatoid arthritis

Fig. 4

a The efficacy of kaempferol on bFGF-induced human FLS proliferation was measured by MTS assay. b AP-1 transactivation and Cox-2 promoter activities were measured in RSK2+/+ and RSK2−/− MEF by transfection of pAP-1-luciferase (top graph) and pCox-2 promoter-luciferase (bottom graph) reporter plasmids as indicated. c Effects of bFGF-induced cell migration in RSK2+/+ and RSK2−/− MEFs. The migrated area was quantified by measuring the uncovered area of the wound using Image J (Ver. 1.6). d Efficacy of kaempferol on the cell migration of MH7A and human FLSs. The migrated area (graphs) was quantified by measuring the uncovered area of the wound using Image J (Ver. 1.6). e Efficacy of kaempferol on MMP-9 and MMP-2 activity was analyzed by gelatin zymography using the indicated culture supernatants of human FLSs. f Representative photographs for kaempferol specificity on FGFR3 phosphorylation at Tyr724 induced by bFGF stimulation in MH7A cells. PKC412, an FGFR3 inhibitor; U0126, an MEK inhibitor. Data were obtained from three independent experiments. Each indicated area in the immunocytofluorescence confocal image (×400) was magnified (×630). Scale bars, 20 μm. a–d Data were obtained from three independent experiments, and values are represented as means ± SEM. *p < 0.05; **p < 0.01; ***p < 0.001 by Student’s t-test

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