Fig. 2: PFKFB3 expression in HCC cells promoted tumor growth in vitro and in vivo. | Cell Death & Disease

Fig. 2: PFKFB3 expression in HCC cells promoted tumor growth in vitro and in vivo.

From: PFKFB3 blockade inhibits hepatocellular carcinoma growth by impairing DNA repair through AKT

Fig. 2: PFKFB3 expression in HCC cells promoted tumor growth in vitro and in vivo.The alternative text for this image may have been generated using AI.

a Clone formation of SMMC7721-shPFKFB3 cells and Huh7-PFKFB3 cells compared with their vector control. PFKFB3 promoted the cells clone formation in vitro. b CCK8 assay for cell proliferation of SMMC7721-shPFKFB3 cells and Huh7-PFKFB3 cells compared with their vector control. PFKFB3 promoted the liver cancer cells proliferation in vitro. c Comparison of tumor sizes for the SMMC7721-shPFKFB3 cells group and SMMC7721-shVector cells group (45.3 ± 14.9 mm3 vs. 201.9 ± 88.6 mm3; p = 0.02), and comparison of tumor sizes for the Huh7-PFKFB3 cells group and Huh7-Vector cells group (825.6 ± 217.9 mm3 vs. 467.8 ± 221.9 mm3; p = 0.033). d Representative immunohistochemistry of liver cancer for the expression of Ki67 from Balb/c nu/nu mice orthotopically implanted with SMMC7721 or Huh7 cells. Ki67 expressed higher in PFKFB3 high expression tumor. (Magnification ×200). e Representative TUNEL fluorescence of liver cancer from Balb/c nu/nu mice orthotopically implanted with SMMC7721 or Huh7 cells. The apoptosis rate of cells was higher in PFKFB3 low expression tumor. (Magnification ×200). *p < 0.05, **p < 0.01

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