Fig. 6: Suppression of brain inflammatory aggravation and infarct expansion in PSI animals by blocking HMGB1 function. | Cell Death & Disease

Fig. 6: Suppression of brain inflammatory aggravation and infarct expansion in PSI animals by blocking HMGB1 function.

From: Alarmin HMGB1 induces systemic and brain inflammatory exacerbation in post-stroke infection rat model

Fig. 6

HMGB1 A box (5 μg/kg) or HPep1 (5 μg/kg) was administered intranasally 3 h prior to LPS administration (100 μg/kg, i.p.) at 24 h post-MCAO or LPS was preincubated with HMGB1 A box for 6 h before administration to MCAO as shown in Fig. 5a.a Levels of inflammatory markers in cortex penumbra of the ischemic hemisphere (Fig. 3a) were measured at 2 days post-MCAO by immunoblotting. Results are presented as mean ± SEM (n = 4). b, c Representative images of infarctions in coronal brain sections obtained were assessed at 2 days post-MCAO by TTC staining (b) and mean infarction volumes are presented as mean ± SEM (n = 6–8) (c). Sham sham-operated rats (n = 3), MCAO treatment-naive MCAO rats (n = 8), MCAO+LPS LPS-treated MCAO rats (n = 9), MCAO+LPS+A box A box-treated MCAO+LPS rats (n = 7), MCAO+LPS+HPep1 HPep1-treated MCAO+LPS rats (n = 4), MCAO+LPS+Pre-A box MCAO+LPS rats treated with LPS after preincubation with A box (n = 3). **p < 0.01 vs. LPS-treated MCAO group

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