Fig. 1: CircHIPK3 is frequently upregulated in CRC and predicts poor prognosis. | Cell Death & Disease

Fig. 1: CircHIPK3 is frequently upregulated in CRC and predicts poor prognosis.

From: RETRACTED ARTICLE: CircHIPK3 promotes colorectal cancer growth and metastasis by sponging miR-7

Fig. 1: CircHIPK3 is frequently upregulated in CRC and predicts poor prognosis.The alternative text for this image may have been generated using AI.

a The genomic loci of the HIPK3 gene and circHIPK3. Red arrow the back-splicing of HIPK3 exon2 confirmed by Sanger sequencing. b The abundance of circHIPK3 and HIPK3 mRNA was, respectively, assessed by qRT-PCR in HCT116 cell treated with Actinomycin D at the indicated time points. c qRT-PCR analysis of circHIPK3 and HIPK3 mRNA after treatment with RNase R in HCT116 cell. d Fluorescence in situ hybridization assay was conducted to determine the subcellular localization of circHIPK3. e, f qRT-PCR for the expression of circHIPK3 in CRC cell lines and tissues. g CRC patients with high circHIPK3 expression (n = 89) had lower overall survival rates than patients with low circHIPK3 expression (n = 89) did (p < 0.001). Data were represented as means ± S.D. of at least three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001

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