Fig. 2: β 2 spectrin expression correlated with hepatocyte differentiation in DEN-induced rat model of liver fibrosis and HCC. | Cell Death & Disease

Fig. 2: β 2 spectrin expression correlated with hepatocyte differentiation in DEN-induced rat model of liver fibrosis and HCC.

From: β2 spectrin-mediated differentiation repressed the properties of liver cancer stem cells through β-catenin

Fig. 2: β 2 spectrin expression correlated with hepatocyte differentiation in DEN-induced rat model of liver fibrosis and HCC.

a Quantification of β 2 spectrin mRNA transcript in whole liver homogenates from control and DEN-treated rats by real-time RT–PCR. b Real-time RT–PCR determined β 2 spectrin mRNA transcript in hepatocytes isolated from control or DEN-treated rat. The results showed that the transcripts of β 2 spectrin were reduced in hepatocytes during hepatocarcinogensis. c Real-time RT–PCR quantification of liver-specific genes (albumin, HNF4α, HNF1α, G6PC) and α fetoprotein in DEN-treated rats. d Immunohistochemical staining determined the expressions of β2SP, liver-specific genes (albumin, HNF4α), and liver cancer stem cell markers (CD133, EpCAM, CK19) in DEN-treated rats. Magnification, ×200. All the data were means ± SEM of three independent experiments (**P < 0.01, *P < 0.05)

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