Fig. 3: β2SP suppressed the growth of HCC cells (SUN398 and Huh7 cells) in vitro. | Cell Death & Disease

Fig. 3: β2SP suppressed the growth of HCC cells (SUN398 and Huh7 cells) in vitro.

From: β2 spectrin-mediated differentiation repressed the properties of liver cancer stem cells through β-catenin

Fig. 3: β2SP suppressed the growth of HCC cells (SUN398 and Huh7 cells) in vitro.The alternative text for this image may have been generated using AI.

The plasmids expressing β2SP were transfected into SUN398 and Huh7 cells. 48 h later, the cells were collected for further studies. a Quantitative RT–PCR determined β2SP mRNA in HCC cells transfected with the vector expressing β2SP cDNA or the control; b Western blot analysis determined β2SP protein in HCC cells transfected with the vector expressing β2SP cDNA or the control; c the percentages of cell distribution in each phase were determined in transfected HCC cells by flow cytometry. Percentages of cell distribution in each phase were shown on the histogram. d the expression patterns of cell cycle regulatory proteins were analyzed by western blot e the proliferation of HCC cells was evaluated by Cell Counting Kit-8 (CCK8). All the data were means ± SEM of three independent experiments (**P < 0.01, *P < 0.05)

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