Fig. 6: SOCS2 regulates EMT of lung adenocarcinoma cells through STAT3 and STAT5 signalling pathway. | Cell Death & Disease

Fig. 6: SOCS2 regulates EMT of lung adenocarcinoma cells through STAT3 and STAT5 signalling pathway.

From: Suppressor of cytokine signalling-2 limits IGF1R-mediated regulation of epithelial–mesenchymal transition in lung adenocarcinoma

Fig. 6: SOCS2 regulates EMT of lung adenocarcinoma cells through STAT3 and STAT5 signalling pathway.The alternative text for this image may have been generated using AI.

a The expression of STAT3, pSTAT3, STAT5, and pSTAT5 were analysed in A549-SOCS2 and SPC-A1-SOCS2 cells by western blotting. b Immunoblotting analyses of STAT3, pSTAT3, STAT5, and pSTAT5 in A549 and SPC-A1 cells in the presence of 200 ng/μL IGF1, harbouring SOCS2 plasmid or not. Representative immunoblots of indicated proteins are shown. The histogram (right panel) represents a densitometric analysis performed to quantify the relative intensity of bands detected by western blotting. Data are represented as the mean ± SEM. *p < 0.05; **p < 0.01; ***p < 0.001. c Coimmunoprecipitation of IGF1R with STAT3 or STAT5 using protein lysates from A549 cells with the increase of SOCS2 expression. GAPDH was used as a loading control. d Schematic diagram of the regulatory mechanism of SOCS2 on IGF1R/STAT signalling pathway in lung adenocarcinoma

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