Fig. 1: HCC-CAFs attract neutrophils via the SDF1a/CXCR4 pathway.

a HCC samples were stained with CD66b and α-SMA antibodies. Hepatoma samples were divided into two groups according to the density of α-SMA density: high density (n = 10) and low density (n = 10). Correlation between levels of α-SMA and CD66b were analyzed, **P < 0.01. b, c Neutrophils isolated from normal donor peripheral blood samples were serum-starved and then allowed to migrate for 3 h toward CM from HCC-CAFs. b One representative experiment is shown. c The data indicate the mean ± SEM of three independent experiments, **P < 0.01. d, e Neutrophils isolated from normal donor peripheral blood samples were serum-starved and then allowed to migrate for 3 h toward HCC-CAFs CM in the presence or absence of CXCR4 or SDF1a blocking antibodies. d One representative experiment is shown. e The data indicate the mean ± SEM of three independent experiments, **P < 0.01