Fig. 5 | Cell Death & Disease

Fig. 5

From: Cancer-associated fibroblasts induce PDL1+ neutrophils through the IL6-STAT3 pathway that foster immune suppression in hepatocellular carcinoma

Fig. 5

IL6-STAT3 signaling is crucial for the activation of neutrophil.a Neutrophils were cultured with IL6 or HCC-CAFs CM in the presence or absence of aIL6. Total RNA was extracted from neutrophils and real-time PCR was performed to determine them RNA levels of IL8, CCL2, and TNFa, **P < 0.01, *P < 0.05. b Neutrophils were cultured with IL6 or HCC-CAFs CM in the presence or absence of aIL6. The CD66b, PDL1, and CD62L expression in neutrophils was determined by flow cytometry, **P < 0.01, *P < 0.05. c, d Neutrophils were cultured with IL6 or HCC-CAFs CM in the presence or absence of aIL6, and the phosphorylation level of STAT3 in neutrophils was analyzed by flow cytometry. c One representative experiment is shown. d The data indicate the mean ± SEM of three independent experiments, *P < 0.05

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