Fig. 5 HMGB1 was transactivated by β-catenin/TCF4 complex. | Cell Death & Disease

Fig. 5 HMGB1 was transactivated by β-catenin/TCF4 complex.

From: Wnt signaling induces radioresistance through upregulating HMGB1 in esophageal squamous cell carcinoma

Fig. 5 HMGB1 was transactivated by β-catenin/TCF4 complex.

a qRT-PCR analysis of chromatin modifiers’ fold changes after the inhibition of Wnt signaling. rECA109 and rKyse150 cells were treated with iCRT14 (25 μM for 24 h) and analyzed for expression of chromatin modifiers using qRT-PCR. b qRT-PCR analysis of HMGB1 and CHD4. 24 h after treatment of WNT1 protein, qRT-PCR was performed to analyze the expression of HMGB1 and CHD4. Mean ± SD, N = 3, *P < 0.05, **P < 0.01. c Western blotting analysis of HMGB1. Western blottings were performed to analyze the expression levels of HMGB1 after WNT1 or iCRT14 treatment with or without IR. d ChIP assay of rKyse150 cells. β-catenin/TCF4 transcriptional complex occupies HMGB1 promoter. ChIP amplicons (1–8) were detected by ChIP-PCR

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