Fig. 1: Trib2 is dispensable for NH9 initiated transformation of HSPCs.

a CFC assay of WT and Trib2^−/− HSPCs retrovirally transduced with MigR1 or NH9 expressing vectors. Graph shows total colony number at CFC1-4. Data are mean of duplicate cultures ± SD; representative of 2 independent experiments. b Gene expression analysis in WT and Trib2^−/− HSPCs retrovirally transduced with MigR1 or NH9 at 48 h post transduction. Data are means of 3 biological samples (from 1 to 2 mice per biological replicate) ± SD and representative of 2 independent experiments. *P < 0.05, **P < 0.005, ***P < 0.001, using unpaired t-test. c, d Representative dot plots of the CD11b+c-Kit+ fraction (c) and c-Kit expression histograms (d) of WT and Trib2^−/− NH9 cells transformed by CFC assay (top panels) or LC condition (bottom panels), gated through GFP+DAPI-Lin(CD4, CD8, B220, Ter119)-Sca1- gates. e Graphed percentages are average of 3 independent measurements, as shown (c, d) ±SD. *P ≤ 0.05, **P < 0.005, using unpaired t-test. f Representative flow cytometry analyses identifying the L-GMP population in WT and Trib2^−/− NH9 cells transformed by CFC assay (top panels) or LC condition (bottom panels), gated through GFP+DAPI-Lin(CD3, CD4, CD8, CD19, B220, Ter119, Gr1)-CD127- and Sca1-c-Kit+(LK) gates. g Graph shows percentages of the L-GMP cells, as shown (f), in the GFP+DAPI-Lin-fraction. Data are mean of 3 independent measurements ± SD. *P ≤ 0.05, **P < 0.005, using unpaired t-test