Fig. 7: Re-introduction of Trib2 in Trib2-/- NH9 cells re-establishes an apoptotic and cell cycle response to drug treatment.
From: A Trib2-p38 axis controls myeloid leukaemia cell cycle and stress response signalling
Flow cytometric analysis of apoptotic levels by means of AnnexinV/DNA DAPI (a), Cell cycle Ki67/DNA DAPI (b), and mitotic index by means of p-HH3/DNA DAPI staining (c) in WT and Trib2-/- NH9 MigR1 NGFR/NGFR Trib2 transduced cells, after 24 h DNR treatment. d Shows graphed percentages of Live (AnnexinV-/DNA DAPI-) and Late Apoptotic (AnnexinV+/DNA DAPI+) cells, as measured (a). Data are representative of 4 independent experiments, graphs show mean ± SD. *P < 0.05, **P < 0.005, ***P < 0.001, using unpaired t-test. e Graphed percentages of p-HH3+ cells, as measured (c). Data are representative of 2 independent experiments, graphs show mean ± SD. **P < 0.005 using unpaired t-test
