Fig. 2: Characterisation of hiPSCs harbouring the A8344G mutation of mtDNA. | Cell Death & Disease

Fig. 2: Characterisation of hiPSCs harbouring the A8344G mutation of mtDNA.

From: ATOH1/RFX1/RFX3 transcription factors facilitate the differentiation and characterisation of inner ear hair cell-like cells from patient-specific induced pluripotent stem cells harbouring A8344G mutation of mitochondrial DNA

Fig. 2: Characterisation of hiPSCs harbouring the A8344G mutation of mtDNA.

a The proportion of mtDNA with A8344G mutation was quantified by pyrosequencing of MERRF iPSCs (M1-iPSCs and M2-iPSCs). b The reactive oxygen species (ROS) levels of M1-iPSCs and M2-iPSCs were higher than that of M1Ctrl, as indicated by flow cytometry. *p < 0.05, **p < 0.01, ***p < 0.001, N = 3. c Quantitative RT-PCR (qRT-PCR) demonstrated that the expression level of the antioxidant enzyme gene CAT in M1- and M2-iPSCs was significantly higher than that in M1Ctrl-iPSCs. *p < 0.05, **p < 0.01, ***p < 0.001, N = 3

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