Fig. 4: Differentiation of HC-like cells from human iPSCs (hiPSCs) through the ATOH1/RFX1/RFX3 TF method. | Cell Death & Disease

Fig. 4: Differentiation of HC-like cells from human iPSCs (hiPSCs) through the ATOH1/RFX1/RFX3 TF method.

From: ATOH1/RFX1/RFX3 transcription factors facilitate the differentiation and characterisation of inner ear hair cell-like cells from patient-specific induced pluripotent stem cells harbouring A8344G mutation of mitochondrial DNA

Fig. 4: Differentiation of HC-like cells from human iPSCs (hiPSCs) through the ATOH1/RFX1/RFX3 TF method.

a Schematic of the HC differentiation protocol with lentiviral infections (MYO7AmCherry reporter gene, ATOH1, RFX1/RFX3 and ATOH1/RFX1/RFX3) on day 21. b The number of MYO7AmCherry-positive cells in the ATOH1/RFX1/RFX3 and ATOH1 conditions was higher than that in the control (Ctrl) condition. The number of MYO7AmCherry-positive cells in the ATOH1/RFX1/RFX3 and ATOH1 conditions was higher than those in the RFX1/RFX3 and ATOH1 conditions. Positive cells were counted by randomly selecting five fields in each experimental condition. Data are presented as mean ± SD, **p < 0.01, ***p < 0.001

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