Fig. 3: Expression of UPR and functional genes in EOCs. BMNCs from non-diabetic (NDM) and diabetic (DM) mice were cultured in EGM-2 medium for 7 days to generate EOCs. | Cell Death & Disease

Fig. 3: Expression of UPR and functional genes in EOCs. BMNCs from non-diabetic (NDM) and diabetic (DM) mice were cultured in EGM-2 medium for 7 days to generate EOCs.

From: Reduction of Endoplasmic Reticulum Stress Improves Angiogenic Progenitor Cell function in a Mouse Model of Type 1 Diabetes

Fig. 3

a Day 7 EOCs were harvested and expression of ER stress markers was determined by qRT-PCR. Data are presented as mean ± SD (n = 4 mice per group). b Protein levels of CHOP and cleaved caspase-3 (c-casp-3) in EOCs were examined by western blot analysis and quantified using densitometry (mean ± SD, n = 3 mice per group). c Expression of ER stress and functional genes in EOCs was measured by qRT-PCR (mean ± SD, n = 5–7 mice per group). d The mRNA levels of selected UPR and functional genes in EOCs assessed by PCR array (mean ± SD, n = 3 mice per group). *P < 0.05, **P < 0.01 (Student’s t-test)

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