Fig. 4: Inhibition of ER stress by ex vivo chemical chaperone treatment enhances the functionality of diabetic EOCs. | Cell Death & Disease

Fig. 4: Inhibition of ER stress by ex vivo chemical chaperone treatment enhances the functionality of diabetic EOCs.

From: Reduction of Endoplasmic Reticulum Stress Improves Angiogenic Progenitor Cell function in a Mouse Model of Type 1 Diabetes

Fig. 4

BMNCs from non-diabetic (NDM) and diabetic (DM) mice were cultured in EGM-2 medium with or without 20 μmol/l of 4-PBA for 7–8 days. a EOCs from 1-month-diabetic mice and their non-diabetic controls (white circles) were counted. b EOC quantification after 6 days of culture (n = 3 mice per group). c EOCs were counted. d EOC quantification after 6 days of culture (n = 3-6 mice per group). e In vitro migration assay of EOCs toward VEGF. f The migrated cells were stained with DAPI and counted. The results are expressed as fold of NDM control (four mice per group). Scale bars, 100 μm. All data are presented as mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001, Student’s t-test (a, b) and one-way ANOVA with Tukey’s post hoc test (c–f)

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