Fig. 2: FGFR2b has a sequential role in distinct steps of the keratinocyte differentiation. | Cell Death & Disease

Fig. 2: FGFR2b has a sequential role in distinct steps of the keratinocyte differentiation.

From: Role of FGFR2b expression and signaling in keratinocyte differentiation: sequential involvement of PKCδ and PKCα

Fig. 2

HaCaT pBp and HaCaT pBp-FGFR2b clones grown up to confluence or post-confluence were left untreated or stimulated with FGF7 as reported in Materials and methods section. a Western blot analysis shows that in confluent conditions the levels of K1, DSG1, and INV are increased upon FGF7 stimulation particularly in pBp-FGFR2b cells, while no appreciable levels of FIL and LOR are visible in these cells. In addition, the expression of β1-integrin appears strongly decreased in HaCaT pBp-FGFR2b in response to ligand stimulation. b In post-confluence conditions all the early/intermediate differentiation markers appear increased making their FGF7-induced modulation less evident compared to the corresponding confluent cells. In contrast, FIL and LOR are detectable and they appear up-regulated in response to FGF7 stimulation, particularly in cells overexpressing FGFR2b. The equal loading was assessed with anti-ACTIN and anti-TUBULIN antibodies. For densitometric analysis the values from a representative of three independent experiments are reported

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