Fig. 1: Rnd1 expression is downregulated in HCC and predicts poor prognosis. | Cell Death & Disease

Fig. 1: Rnd1 expression is downregulated in HCC and predicts poor prognosis.

From: The Rho GTPase Rnd1 inhibits epithelial–mesenchymal transition in hepatocellular carcinoma and is a favorable anti-metastasis target

Fig. 1: Rnd1 expression is downregulated in HCC and predicts poor prognosis.The alternative text for this image may have been generated using AI.

a Rnd1 mRNA expression in 20 paired HCC tissues (T) and adjacent non-tumor liver tissues (ANLTs). Expression level of Rnd1 was normalized to GAPDH. Fold changes were analyzed using the formula: 2−(∆∆ CT [HCC/ANLT]). Red line indicates fold change of Rnd1 equal to 2. b Protein expression of Rnd1 in select HCC tissues (T; n = 8) and adjacent non-tumor liver tissues (ANLTs; n = 8). Densitometry of western blot in panel. Data represent means ± S.D. of three independent experiments, significant difference was tested by Student’s t-test. **P < 0.01, ***P < 0.001. c Representative IHC images of Rnd1 expression in HCC tissues (T) and adjacent non-tumor liver tissues (ANLTs). Scale bar: 100 μm (upper), 20 μm (lower). Rnd1 expression was quantitated based on integrated optical density (IOD). Results were expressed as mean ± S.D., n = 67 patients. Significant difference was tested by Student’s t-test. ***P < 0.001. d Representative IF images of Rnd1 expression in HCC tissues (T) and adjacent non-tumor liver tissues (ANLTs); boundaries between T and ANLT tissue are indicated by a dashed line. Scale bar: 100 μm. e Kaplan–Meier analysis indicates that low expression of Rnd1 is correlated with poor overall survival and disease-free survival in 67 cases of HCC patients. **P < 0.01

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