Fig. 2: Attenuated Rnd1 expression promotes the migration and invasion of HCC cells in vitro and in vivo. | Cell Death & Disease

Fig. 2: Attenuated Rnd1 expression promotes the migration and invasion of HCC cells in vitro and in vivo.

From: The Rho GTPase Rnd1 inhibits epithelial–mesenchymal transition in hepatocellular carcinoma and is a favorable anti-metastasis target

Fig. 2

a, b Western blot and Real-time PCR analysis of the Rnd1 protein and mRNA expression, respectively, in one normal liver cell line (L02) and five HCC cell lines (Huh7, HepG2, MHCC97L, MHCC97H, and HCCLM3). Panels represent mean ± S.D. (n = 3) from three independent experiments plated in triplicate. Significance was determined using one-way ANOVA. ***P < 0.001. c The impact of Rnd1 knockdown and overexpression on cell migration and invasion, as evaluated by transwell assays with or without Matrigel. Representative images of migrated or invasive cells are shown. Scale bar: 100 μm. The number of cells passed through the membrane was counted and compared in the graphs below, the results are expressed as the mean ± S.D. (n = 3) of three independent experiments, significance was determined using one-way ANOVA. **P < 0.01. d Representative fluorescence images of lungs derived from orthotopic xenograft tumor models. e Representative HE staining images for lung metastasis. Scale bar: 100 μm (upper), 20 μm (lower). The percentage of mice with or without metastatic nodules in the lungs was calculated and compared (5 mice in each group). Significance was determined using χ2 test. **P < 0.01

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