Fig. 5: Attenuated Rnd1 expression activates Raf/MEK/ERK signaling by enhancing the activity of RhoA. | Cell Death & Disease

Fig. 5: Attenuated Rnd1 expression activates Raf/MEK/ERK signaling by enhancing the activity of RhoA.

From: The Rho GTPase Rnd1 inhibits epithelial–mesenchymal transition in hepatocellular carcinoma and is a favorable anti-metastasis target

Fig. 5

a The interaction diagram of Rnd1 with RhoA, Raf/MEK/ERK, and EMT related molecules, as analyzed by STRING 10.0. b Western blot analysis of RhoA-GTP pull-down assay, indicating the degree of RhoA activation, and protein expression of ROCK1 and P-MYPT(T696), in Huh7-shRnd1 cells, MHCC97H-Rnd1 cells, and their corresponding wild type and control cells. c Changes in protein expression of Rnd1, RhoA-GTP, ROCK1, and P-MYPT (T696) in HCC cells with modulation of Rnd1 treated with RhoA siRNA, ectopic expression of RhoA, or stimulated with S1P. d Changes in protein expression of P-C-Raf (S338), P-MEK (Ser217/221), and P-ERK (T202/Y204) in HCC cells with modulation of Rnd1 treated with RhoA siRNA, ectopic expression of RhoA, or stimulated with S1P. All data for the densitometric analysis of the western blots are presented as mean ± S.D. (n = 3) of three independent experiments. Significance was determined using two-way ANOVA. *P < 0.01, ***P < 0.001

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