Fig. 1: A high level of protein O-GlcNAcylation is associated with chemoresistance in cancer cell lines. | Cell Death & Disease

Fig. 1: A high level of protein O-GlcNAcylation is associated with chemoresistance in cancer cell lines.

From: O-GlcNAc elevation through activation of the hexosamine biosynthetic pathway enhances cancer cell chemoresistance

Fig. 1

a MCF-7, HL60, Hela and SMMC-7721 cells were treated with the indicated dose of DOX or CPT for 24 h. The cell viability was assessed through the MTS assay. b and c Cells with intrinsic and acquired chemoresistance contain elevated levels of O-GlcNAc-modified proteins compared with sensitive cells. The cellular O-GlcNAcylation and expression of indicated proteins in parental and resistant cancer cell lines were analysed by immunoblotting analysis. d O-GlcNAcylation is upregulated in both parental and resistant cancer cells after transient treatment with DOX. The indicated cells were treated with DOX (0.1 μM for MCF-7, 1 μM for MCF-7/ADR and SMMC-7721) for 6 h. Whole-cell lysates were obtained after treatment and analysed by immunoblotting. e The indicated cell lines were transfected with scrambled siRNA (siRNA-Scr) or OGT siRNA (siOGT) for 48 h and then treated with 1 μM DOX or 0.5 μM CPT for an additional time. The protein levels after treatment with DOX or CPT for 6 h were examined by immunoblotting. The cell viability after treatment with DOX or CPT for 24 h was assessed through an MTS assay. f MCF-7 cells were transfected with control (pcDNA) or OGT-overexpressing vector (OGT) for 48 h and then treated with 1 μM DOX or 0.5 μM CPT for an additional time. The protein levels after treatment with DOX or CPT for 6 h were examined by immunoblotting. The cell viability after treatment with DOX or CPT for 24 h was assessed through an MTS assay. The data represent the means ± SEM, N = 3, *p < 0.05, **p < 0.01

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