Fig. 5: Upregulated Lnc-Tim3 leads to nuclear localization of Bat3 and enhances p300-dependent p53 and RelA transcriptional activity. | Cell Death & Disease

Fig. 5: Upregulated Lnc-Tim3 leads to nuclear localization of Bat3 and enhances p300-dependent p53 and RelA transcriptional activity.

From: Long non-coding RNA Lnc-Tim3 exacerbates CD8 T cell exhaustion via binding to Tim-3 and inducing nuclear translocation of Bat3 in HCC

Fig. 5

a, b Jurkat T cells were transfected with indicated lentiviral particles (Tim-3 wild type, Lnc-Tim3 wild type or Δ226-277) and treated with anti-TCR/anti-CD28 beads and PMA-ionomycin. After 6 h, cytosolic and nuclear fractions were prepared from extracts. Nuclear localization of Bat3 and p300 was determined by western blot (a) and confocal scanning laser microscopy (b). The white arrow and circle represent Nuclei. Representative of three experiments. c, d Jurkat T cells were transfected with indicated lentiviral particles (Tim-3 wild type, Lnc-Tim3 wild type or Δ226-277) and treated with anti-TCR/anti-CD28 beads and PMA-ionomycin. Nuclear localization of p300 and p53 (c) or RelA (d) was determined by confocal scanning laser microscopy. The white arrow and circle represent Nuclei. Representative of three experiments. e Jurkat T cells were transfected with indicated lentiviral particles (Tim-3 wild type, Lnc-Tim3 wild type or Δ226-277) and treated with anti-TCR/anti-CD28 beads and PMA-ionomycin. Protein levels of Ac-p53, p21, MDM2, and Bcl-2 were determined by western blot. Representative of three experiments

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