Fig. 8: Murine MSCs pretreated with LDN57444 alleviated ConA-induced liver injury. | Cell Death & Disease

Fig. 8: Murine MSCs pretreated with LDN57444 alleviated ConA-induced liver injury.

From: The deubiquitinating enzyme UCHL1 negatively regulates the immunosuppressive capacity and survival of multipotent mesenchymal stromal cells

Fig. 8

Mice were intravenously injected with ConA (20 mg/kg), untreated, DMSO-pretreated and LDN57444-pretreated MSCs labeled with CFSE or not were transfused immediately. 8 h later, livers and serum were sampled. a Percentages of CFSE-labeled MSCs (CFSE+ MSCs) in the livers were calculated. b The iNOS expression of CFSE+ MSCs was determined by flow cytometry and MFI of iNOS in CFSE+ MSCs were shown. c Cells were harvested and stained with annexin V, and the percentages of annexin V+ cells of CFSE+ MSCs were shown. d Hematoxylin and eosin staining of liver sections. N: necrosis area. e Percentages of necrosis area in the livers were calculated. f Serum levels of ALT were measured. g Absolute numbers of MNCs, CD4+ and CD8+ T cells in liver tissues were determined by flow cytometry. Values are shown as mean ± S.E.M. and statistical significance indicated as *P < 0.05, **P < 0.01, and ***P < 0.001.

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