Fig. 2: CD51+ cells showed PCa CSC properties.
From: p53-dependent CD51 expression contributes to characteristics of cancer stem cells in prostate cancer
CD51+ or CD51− cells sorted from DU 145 and PC-3 cell lines. a Sphere-formation assays showed that the CD51+ cells exhibited higher sphere-forming capacities than the corresponding CD51− cells. Scale bar: 100 μm. b CD51, Nestin, and Sox2 immunoblots from CD51+ and CD51− cells in DU 145 and PC-3. c Tumor formation in vivo was induced by the subcutaneous injection of PCa cells (dose of 2 × 106) into 6-week-old SCID mice (n = 6). Tumors on the left line were formed by CD51+ cells, while tumors on the right line were formed by CD51− cells. d RT-qPCR was used to evaluate the expression of the stem-related genes Nestin, Nanog, and Sox2 at the mRNA level in CD51+ and CD51− cells. e Flow cytometric analysis of the co-expression of CD51-FITC with the CD44-PerCP-Cy5.5 antibody in two cell lines. f Flow cytometric analysis of ALDH activation in CD51+ and CD51− cells. Error bars represent the standard deviation (SD) of data obtained from three independent experiments (**p < 0.01, ***p < 0.001)
