Fig. 4: E2F3 is a direct downstream target of HOXB9.
From: HOXB9 promotes endometrial cancer progression by targeting E2F3
a The endometrial cancer cell line Ishikawa was knocked down with two different HOXB9 small interfering RNA (siRNA), and cell lysates were analyzed by western blot with the indicated antibodies. b The endometrial cancer cell line Ishikawa was transfected with Flag-HOXB9 or empty vector plasmid, and cell lysates were analyzed by western blot with the indicated antibodies. c The endometrial cancer cell line Ishikawa was knocked down with two different HOXB9 siRNA, and E2F3 and HOXB9 mRNA expression levels were analyzed by real-time PCR. d ChIP assays were performed using control IgG or anti-Flag antibody in Ishikawa cells. The binding of HOXB9 on promoters of E2F3 and the negative control Actb genes were analyzed by real-time PCR. Data were presented as mean ± SEM from three independent experiments. The statistical analyses were performed by Student’s t-test, ***P < 0.001
