Fig. 6: C2EIP and PTCH2 are co-expressed at the intracellular membrane. | Cell Death & Disease

Fig. 6: C2EIP and PTCH2 are co-expressed at the intracellular membrane.

From: Interaction of the primordial germ cell-specific protein C2EIP with PTCH2 directs differentiation of embryonic stem cells via HH signaling activation

Fig. 6: C2EIP and PTCH2 are co-expressed at the intracellular membrane.The alternative text for this image may have been generated using AI.

a Interaction between PTCH2 and C2EIP proteins was observed by GSTpull-down test. IgG antibody was used as a negative control. b Indirect immunofluorescence results indicate that PTCH2 protein is localized in cell membranes, Scale bar: 30 μm. SSEA-1was used as acell membrane marker. c Indirect immunofluorescence results indicate that PTCH2 and C2EIP colocalize at cell membranes, Scale bar: 20 μm. d Co-immunoprecipitation from PGC detected an interaction between GST-C2EIP and PTCH2 proteins. IgG antibody was used as negative control. e Western blot analysis showed that GST-C2EIP and PTCH2 are expressed in the cytoplasm of PGC

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