Fig. 5: Suppression of PP2A reduces IR sensitivity of stem cells. | Cell Death & Disease

Fig. 5: Suppression of PP2A reduces IR sensitivity of stem cells.

From: Transient PP2A inhibition alleviates normal tissue stem cell susceptibility to cell death during radiotherapy

Fig. 5

ES and ED cells were treated with Calyculin A (Cal A), control siRNA (siCtrl), or PP2A siRNA (siPP2A) or left untreated. Error bars indicate SD; *p < 0.05; **p < 0.01; n.s. = not significant. Three independent experiments were performed. a1 ES and ED cells were treated with Cal A, irradiated with 6 Gy and apoptosis analyzed after 8 and 12 h by Annexin V labeling. a2 ES and ED cells were treated with siPP2A, irradiated with 6 Gy and apoptosis analyzed after 8 and 12 h by Annexin V labeling. b Human medulloblastoma cells (Daoy HTB-186) were treated with Cal A or left untreated. Cells were irradiated with 6 Gy and apoptosis analyzed after 16 h by Annexin V labeling. c Murine glioblastoma cells (GL261) were treated with Cal A or left untreated. Cells were irradiated with 6 Gy and apoptosis analyzed after 16 h by Annexin V labeling. d Clonogenic survival of ES cells was analyzed after the indicated doses of irradiation

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