Fig. 4: Knockdown of TFEB impairs lysosomal activation by docetaxel. | Cell Death & Disease

Fig. 4: Knockdown of TFEB impairs lysosomal activation by docetaxel.

From: Docetaxel enhances lysosomal function through TFEB activation

Fig. 4: Knockdown of TFEB impairs lysosomal activation by docetaxel.The alternative text for this image may have been generated using AI.

a, b AGS cells were transfected with scrambled or TFEB siRNA for 48 h and then treated with docetaxel (10 nM) for 12 h. After immunostaining with SQSTM1 (Alexa Fluor 488, green) and LAMP1 (Alexa Fluor 594, red), cells were examined by fluorescence microscopy (scale bar 20 μm). The colocalization of SQSTM1 and LAMP1 was calculated and statistically analyzed. *P < 0.05. c As in (a), at the indicated time, cells were loaded with Magic Red cathepsin B for 15 min. Fluorescence intensity of 10,000 cells per sample was measured by flow cytometry. *P < 0.05

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