Fig. 7: Leptin-accelerated OMA1 destruction depends on phosphorylation of GSK3. | Cell Death & Disease

Fig. 7: Leptin-accelerated OMA1 destruction depends on phosphorylation of GSK3.

From: Leptin increases mitochondrial OPA1 via GSK3-mediated OMA1 ubiquitination to enhance therapeutic effects of mesenchymal stem cell transplantation

Fig. 7

a HEK293T cells transfected with Flag-OMA1 plasmid was pulled down via co-IP analysis with monoclonal anti-flag M2 antibody. GSK3 protein expression was detected by western blot using anti-GSK3 antibody. b, c Phosphorylation of GSK3 (Tyr-216/Tyr-279) was detected by western blot and protein bands from hMSCs-Leppre and hMSCs-Ctrlpre quantified by densitometry. d A schematic illustration showed that leptin protected mitochondrial integrity through the OPA1-dependent pathway, which was due to OMA1 ubiquitination to enhance hMSCs survival and increased the paracrine of hMSCs rescued heart function

Back to article page