Fig. 5: Cdk1 induces G1 checkpoint abrogation.

a Cdk1 protein levels were measured by Western blot 48 h after transfection with siRNAs targeting Cdk1. b Flow cytometry of cells transfected with Cdk1 siRNAs for 24 h, treated with bleomycin for 36 h, then stained with PI. c Flow cytometry of cells transfected with siRCC1 and Flag or Flag-Cdk1 for 24 h and then treated with bleomycin for 36 h. Data representative of three biological replicates are shown. *P < 0.05; **P < 0.01