Fig. 7: Effect of MAPK1/2 inhibition on HSPB1 expression in the whole WT hippocampus and WT astrocytes following KA injection.

a Representative western blot of autophagy-related molecules in the whole hippocampus (Left panels) and isolated astrocytes (Right panels). As compared to vehicle (Veh), U0126 leads to HSPB1-mediated astroglial autophagy in WT mice 7 days after SE (KA/7D). b–c Quantifications of effect of U0126 on HSPB1-mediated astroglial autophagy in the whole hippocampus (b) and isolated astrocytes (c). Open circles indicate each individual value. Horizontal bars indicate mean value. Error bars indicate SEM (*p < 0.05 vs. vehicle, respectively; n = 7, respectively). d Representative photos demonstrating the effect of U0126 on HSPB1 and LAMP1 expression in WT mice 7 days after KA injection. U0126 increases both HSPB1 and LAMP1 expression in astrocytes