Fig. 1: Detection of GX1-receptor expression in co-HUVECs and the effect of GX1 on co-HUVECs migration. | Cell Death & Disease

Fig. 1: Detection of GX1-receptor expression in co-HUVECs and the effect of GX1 on co-HUVECs migration.

From: Novel peptide GX1 inhibits angiogenesis by specifically binding to transglutaminase-2 in the tumorous endothelial cells of gastric cancer

Fig. 1: Detection of GX1-receptor expression in co-HUVECs and the effect of GX1 on co-HUVECs migration.The alternative text for this image may have been generated using AI.

a After co-HUVEC lysates were incubated with different concentrations of biotin-GX1 and biotin-URP (0.2, 0.1, 0.02, and 0.01 mg/ml), one specific binding band at approximately 70 kDa was detected with the biotin-labeled peptide GX1 by Western blotting (indicated by red arrows 1, 2, 3, and 4). b Immunofluorescence staining of the GX1 receptor (red) in HUVECs and co-HUVECs. c Wound healing assay of the migration of co-HUVECs pretreated with or without GX1. Pictures were obtained at a magnification of 200× and 12 h after scratching. ***P < 0.001, n = 4. d Transwell assay examining the effect of GX1 (0.1 mg/ml, 24 h) on co-HUVECs migration. Pictures were captured at a magnification of 200×. The number of migratory cells per field was calculated with ImageJ software. *P < 0.05, n = 4

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