Fig. 3: Expression of TGM2 and co-localization of GX1-receptor and TGM2 in cells and tissue of gastric cancer.

a (panels a and b) Total protein lysates from co-HUVECs, HUVECs, SGC7901 cells and GES cells were incubated with TGM2-antibody and examined by Western blotting. *P < 0.05; **P < 0.01, n = 3. b Three serial sections of gastric cancer tissues were analyzed concurrently by immunohistochemistry staining for GX1-receptor, TGM2 or CD31. c Dual immunofluorescence staining of GX1-receptor (green) and TGM2 (red) in HUVECs and co-HUVECs. The Merged pictures show the overlay of GX1-receptor (green) and TGM2 (red) and the nuclei stained with 40, 6-diamidino-2-phenylindole (DAPI, blue)