Fig. 2: 10e decreased protein levels of c-Flip, HDAC3 and 6 and led to Ku70 acetylation. | Cell Death & Disease

Fig. 2: 10e decreased protein levels of c-Flip, HDAC3 and 6 and led to Ku70 acetylation.

From: HDAC and Ku70 axis- an effective target for apoptosis induction by a new 2-cyano-3-oxo-1,9-dien glycyrrhetinic acid analogue

Fig. 2

A HL-60 cells were treated with the indicated concentrations of 10e for 6 h, then the levels of the indicated proteins were determined using western blot analysis. B HL-60 cells were treated with MS-275 or tubacin at the indicated concentrations for 24 h, then relative levels of the indicated proteins were determined by Western blotting. C, D The whole-cell lysates from HL-60 cells treated with 2 μM 10e for the indicated time were immunoprecipitated with an acetylated lysine antibody, an Ku70 antibody (C) or with an c-Flip antibody (D), and then immunoblotted with an anti-Ku70, an acetylated lysine or an c-Flip antibody as labeled. E The cell lysates from HL-60 cells were immunoprecipitated with an anti-HDAC3 or anti-HDAC6 antibody and then immunoblotted for Ku70, HDAC3, HDAC6 proteins

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