Fig. 3: Downregulation of DNMTs contributed to IFN-γ promoter hypomethylation.
From: MicroRNA-29b/142-5p contribute to the pathogenesis of biliary atresia by regulating the IFN-γ gene
a Levels of DNMTs mRNA were negatively correlated with the mRNA expression of IFN-γ in biliary atresia cases (p < 0.001, r = −0.54, −0.48, −0.42, respectively). b DNMT siRNAs were transfected into Jurkat cells. Levels of DNMTs were detected using qPCR (normalized to β-actin) and western blotting. c The quantitative methylation of the LINE-1, ALU, and SAT2 sequences were analyzed and normalized to ALU-C4 in Jurkat cells. d The methylation of the IFN-γ promoter were detected by bisulfite genomic sequencing, and e levels of IFN-γ were detected using qPCR and normalized to β-actin in Jurkat cells after transfected with DNMT siRNAs. *p < 0.05, **p < 0.01, ***p < 0.001 vs. NC siRNA. ###p < 0.001 vs. DNMTs siRNA. Date represent mean values ± SD from three independent experiments
