Fig. 4: Overexpression of miR-29b/142-5p led to IFN-γ upregulation by targeting DNMTs.
From: MicroRNA-29b/142-5p contribute to the pathogenesis of biliary atresia by regulating the IFN-γ gene
a Mimics of miR-29b/142-5p were transfected into Jurkat cells. Levels of miR-29b/142-5p were detected using qPCR and normalized to U6 levels. The methylation of the IFN-γ promoter were detected by bisulfite genomic sequencing in Jurkat cells. b Levels of DNMTs were detected by qPCR (normalized to β-actin) and western blotting. c Levels of IFN-γ were detected by qPCR and normalized to β-actin. d MiR-29b/142-5p inhibitors were transfected into Jurkat cells. Levels of miR-29b/142-5p were detected using qPCR and normalized to U6 levels. The methylation of the IFN-γ promoter were detected. e Levels of DNMTs were detected by qPCR (normalized to β-actin) and western blotting. f Levels of IFN-γ were detected by qPCR and normalized to β-actin. *p < 0.05, **p < 0.01, ***p < 0.001 vs. miRNA NC mimic or inhibitor. ##p < 0.001 vs. miRNAs mimic or inhibitor. Date represent mean values ± SD from three independent experiments
