Fig. 5: Silence of STC2 in MSCs impaired the activity of HO-1. | Cell Death & Disease

Fig. 5: Silence of STC2 in MSCs impaired the activity of HO-1.

From: Stanniocalcin-2 contributes to mesenchymal stromal cells attenuating murine contact hypersensitivity mainly via reducing CD8+ Tc1 cells

Fig. 5: Silence of STC2 in MSCs impaired the activity of HO-1.

a Cells were co-stained with anti-STC2 (red) and anti-HO-1 (green) and examined under immunofluorescence super-resolution microscopy. Scale bar, 200 μm. b Co-immunoprecipitation of HO-1 and STC2. Proteins were collected from MSCs and immunoprecipitated with anti-HO-1, and the immunoprecipitate was probed with anti-STC2. The controls included 5% of the input HO-1 and STC2 proteins (positive control) and IgG (negative control). The IP lane contains 100% total IP lysate. c MSCshNTC or MSCshSTC2 were co-stained with anti-STC2 (red) and anti-HO-1 (green) and examined under immunofluorescence super-resolution microscopy. Scale bar, 200 μm. d Western blot analysis of STC2 and HO-1 proteins in MSCshNTC and MSCshSTC2. e Culture supernatants of MSCshNTC and MSCshSTC2 were treated with hemoglobin (50 μM/l), and CO levels were determined using an HbCO ELISA kit. Data are presented as the mean ± SEM (n = 4); *p < 0.05 and **p < 0.01

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