Fig. 3: miR-3662 suppresses HCC cell proliferation and induces cell cycle arrest. | Cell Death & Disease

Fig. 3: miR-3662 suppresses HCC cell proliferation and induces cell cycle arrest.

From: MiR-3662 suppresses hepatocellular carcinoma growth through inhibition of HIF-1α-mediated Warburg effect

Fig. 3

a, b CCK-8 assays in SMMC7721 (a) and HCCLM3 (b) cells overexpressing or silencing miR-3662. Three duplicates were performed for each group, and three independent experiments were performed. *P < 0.05 compared with the negative control. c 5-ethynyl-2′-deoxyuridine (EdU) incorporation assays were performed to assess the cell proliferation in miR-3662 overexpressing or silencing SMMC7721 and HCCLM3 cells. EdU positive cells were counted from three random microscopic fields for each well, and these experiments were repeated three times independently. **P < 0.01 compared with the negative control. d Colony formation ability of miR-3662 overexpressing or silencing SMMC7721 and HCCLM3 cells. Three independent experiments were performed for each group. **P < 0.01, ***P < 0.001 compared with the negative control. e Flow cytometry results showing the cell cycle distribution of miR-3662 overexpressing or silencing SMMC7721 and HCCLM3 cells. Three independent experiments were performed for each group. *P < 0.05, **P < 0.01, ***P < 0.001 compared with the negative control. All data are represented as the means ± S.E.M

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