Fig. 3: The effect of S. ven metabolite-mediated fission and fusion on mitochondrial function. | Cell Death & Disease

Fig. 3: The effect of S. ven metabolite-mediated fission and fusion on mitochondrial function.

From: Gene-by-environment interactions that disrupt mitochondrial homeostasis cause neurodegeneration in C. elegans Parkinson’s models

Fig. 3

a Relative mitochondrial uptake of the fluorescent dye tetramethylrhodamine ethyl ester (TMRE) was assessed for the mitochondrial membrane potential (ΔΨm) of C. elegans. Animals (wild-type N2) exposed to S. venezuelae metabolite had a significantly lower ΔΨm then solvent-treated EV control animals at day 8 post-hatching. RNAi knockdown of pink-1 was used as a positive control. Relative TMRE fluorescent intensity was normalized to EV solvent control. These data are presented as mean ± S.E.M.; n = 30 animals per replicate, three independent replicates; one-way ANOVA with Dunnett’s post hoc test for multiple comparisons. *P < 0.05, **P < 0.01. b Representative images of TMRE-stained nematodes following treatment with solvent and metabolite, respectively. The scale bar is 5 μm. c Quantitation of mitochondrial DNA (mtDNA) copy number by qRT-PCR in N2 animals. The metabolite caused a relative decrease of mtDNA copy number in comparison to EV solvent control. cep-1 (RNAi) was included as a positive control. Data represented as mean ± S.E.M.; three biological and three technical replicates; one-way ANOVA with Tukey’s post hoc test for multiple comparisons. *P < 0.05

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