Fig. 4: MiR-17-92 cluster promotes the production of chemokines in keratinocytes via suppressing SOCS1. | Cell Death & Disease

Fig. 4: MiR-17-92 cluster promotes the production of chemokines in keratinocytes via suppressing SOCS1.

From: MicroRNA-17-92 cluster promotes the proliferation and the chemokine production of keratinocytes: implication for the pathogenesis of psoriasis

Fig. 4: MiR-17-92 cluster promotes the production of chemokines in keratinocytes via suppressing SOCS1.

a–c The levels of SOCS1, STAT1, p-STAT1, and IRF-1 in NHKs with different transfections and treatments as indicated were detected by western blotting. β-actin was detected as loading control. Data are representative of three independently performed experiments. d The mRNA levels of CXCL9 and CXCL10 in NHKs with different treatments and transfections as indicated were analyzed using qRT-PCR. Mean ± SD is shown. Data are representative of three individual experiments. *P < 0.05; **P < 0.01; ***P < 0.001; ns not significant. e The culture mediums of NHKs with different treatments and transfections as indicated were analyzed by ELISA to determine the secretion levels of CXCL9 and CXCL10. Mean ± SD is shown. Data are representative of three independently performed experiments. **P < 0.01; ***P < 0.001; ns not significant. f The migrations of CD3+ T cells in response to the culture mediums from NHKs with different treatments and transfections as indicated were evaluated using transwell assay. Mean ± SD is shown. Data are representative of three independently performed experiments. ***P < 0.001; ns not significant

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