Fig. 2: Effects of GCRL1 on gastric cancer cell proliferation, migration, and invasion in vitro. | Cell Death & Disease

Fig. 2: Effects of GCRL1 on gastric cancer cell proliferation, migration, and invasion in vitro.

From: Long noncoding RNA gastric cancer-related lncRNA1 mediates gastric malignancy through miRNA-885-3p and cyclin-dependent kinase 4

Fig. 2

a-f Loss-of-function assay with BGC-823. a Silencing effects of si-GCRL1s examined by qRT-PCR, which were normalized to human GAPDH (n = 3). b MTT assay showing the cell multiplication of BGC-823 cells treated with si-GCRL1s 24, 48, 72, and 96 h after transfection. Results were shown as absorbance at 490 nm (n = 3). c EdU incorporation assay indicating the cell proliferation of BGC-823 cells treated with si-GCRL1s and representative images were shown (scale bars = 50 μm). d Bar graphs of cell multiplication to c upon EdU incorporation assay and results were shown as the ratio of EdU-positive cells to Hoechst-positive cells (n = 5). e Transwell assay assessing the mobility of BGC-823 cells after GCRL1 knockdown and representative images were shown. (scale bars = 100 μm). f Bar graphs of cell mobility to  e upon transwell assay and results were shown as the number of migrated or invaded cells per field (n = 5). Data are expressed as mean ± SD, *p < 0.05 compared to si-nc group (a, b, d, f). g-l Gain-of-function assay with MGC-803. g Enhancements of GCRL1 by pc3.1-GCRL1 examined by qRT-PCR, which were normalized to human GAPDH (n = 3). h MTT assay showing the cell multiplication of MGC-803 cells with GCRL1 overexpression 24, 48, 72, and 96 h after transfection. Results were shown as absorbance at 490 nm (n = 3). i EdU incorporation assay indicating the cell proliferation of MGC-803 cells with GCRL1 overexpression and representative images were shown (scale bars = 50 μm). j Bar graphs of cell multiplication to  i upon EdU incorporation assay and results were shown as the ratio of EdU-positive cells to Hoechst-positive cells (n = 5). k Transwell assay assessing the mobility of MGC-803 cells with GCRL1 overexpression and representative images were shown (scale bars = 100 μm). l Bar graphs of cell mobility to  k upon transwell assay and results were shown as the number of migrated or invaded cells per field (n = 5). Data are expressed as mean ± SD, *p < 0.05 compared to pc3.1 group (g, h, j, l)

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