Fig. 4: GCRL1 directly regulates the expression of miR-885-3p. | Cell Death & Disease

Fig. 4: GCRL1 directly regulates the expression of miR-885-3p.

From: Long noncoding RNA gastric cancer-related lncRNA1 mediates gastric malignancy through miRNA-885-3p and cyclin-dependent kinase 4

Fig. 4

a Expression levels of miR-885-3p in GC tissues, n = 26. b Expression levels of miR-885-3p in gastric cancer cell lines (SGC-7901, MGC-803, BGC-823, and AGS) and normal gastric epithelium cell line GES-1 (n = 3). c Expression levels of miR-885-3p in BGC-823 cells transfected with si-GCRL1s (n = 3). d Expression levels of miR-885-3p in MGC-803 cells with GCRL1 overexpression (n = 3). e Expression levels of miR-885-3p in mice tumor tissues, n = 6 in each group. f Prediction of the binding sites between GCRL1 and miR-885-3p by RNAhybrid. g Dual luciferase reporter assay assessing the direct binding between GCRL1 and miR-885-3p in HEK-293T cells (n = 3). h Detection of GCRL1 by qRT-PCR in BGC-823 cells transfected with Bio-885, Bio-885 mut, or Bio-nc by RNA pull-down assay and results were normalized to human GAPDH (n = 3). Data are expressed as mean ± SD, *p < 0.05 compared to adjacent non-tumor tissues (a), GES-1 (b), si-nc (c), pc3.1 (d), Lv-nc (e), AgomiR-nc (g), or Bio-nc (h). The detections were performed by qRT-PCR and normalized to human U6 (a-e)

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