Fig. 8: SNCG knockdown inhibits the promotion of cancer metastasis by Twist1. | Cell Death & Disease

Fig. 8: SNCG knockdown inhibits the promotion of cancer metastasis by Twist1.

From: Gamma synuclein is a novel Twist1 target that promotes TGF-β-induced cancer cell migration and invasion

Fig. 8

a Hela cells were transfected with lenti-shSNCG-LV3, lenti-shControl-LV3, Twist1-expression plasmid, or the negative control vector, followed by selection of stably transfected cells. Cell lysates were subjected to western blot analysis with indicated antibodies. b The lungs of each mouse were removed and stained in Bouin solution. Pulmonary metastatic nodules were detected. c Scatter dot blots show the numbers of pulmonary metastatic nodules with different size in mice inoculated with HeLa-shControl, HeLa-Twist1-shControl, HeLa-shSNCG, and HeLa-Twist1-shSNCG. The median ± SD was shown. n = 7 mice per group. Δp > 0.05. *p < 0.05

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