Fig. 3: CD24 Knockdown reduced sorafenib resistance.
From: CD24 regulates sorafenib resistance via activating autophagy in hepatocellular carcinoma
a Western blot was used to assay the knockout efficiency of CD24. b Huh7/SR and Hep3B/SR cells were treated with different concentrations (0, 1.5, 3.0, 5.0, 10.0 μM) of sorafenib. After 48 h, the CCK8 assay was used to detect sorafenib sensitivity in the cells. Cells were treated with 1.5 μM sorafenib for 48 h. c Cell proliferation detected by EDU assays. The original magnification was ×400. d Apoptosis detected by AnnexinV-PI assays. Representative images are shown on the top, and quantitative data are on the bottom. Each experiment was performed in triplicate. Data are presented as the means ± s.e.m. and analyzed with Student’s t-test (*P < 0.05, **P < 0.01)
