Fig. 6: CD24 overexpression regulates autophagy activation in sorafenib resistance.
From: CD24 regulates sorafenib resistance via activating autophagy in hepatocellular carcinoma
The level of autophagy was higher in Huh7/SR than Huh7/WT cells, but not in CD24 knockdown cells. a The relative expression level of LC3B and p62 protein was analyzed by western blot. The relative amounts of LC3-II are indicated. b Evaluation of autophagic vacuoles (AVs) by transmission electron microscopy. Cells were cultured with 1.5 μM sorafenib for 48 h then subjected to transmission electron microscopy analysis. The area noted by the red arrows is the autophagic vacuole (AV). Scale bars, 0.5 μm. c The GFP and RFP signals of tandem fluorescent LC3 (RFP-GFP-LC3) show different localization patterns. Cells were transfected with plasmids expressing either tandem fluorescent LC3. Twenty-four hours after transfection, the cells were starved in Hanks balanced salt solution for 2 h, fixed and analyzed by microscopy. Representative images are shown on the left, and quantitative data are on the right. Scale bars, 5 μm. Bar diagram (mean ± s.e.m.) representing the average “yellow only” spot counts/cell (P < 0.05 with Student's t-test). Each experiment was performed in triplicate. The data are presented as the means ± s.e.m. and analyzed with Student’s t-test (*P < 0.05, **P < 0.01)
