Fig. 3: The role of HMGA1 in the proportion of cells in different stages of the cell cycle and its effect on the expression of G1/S transition regulators cyclin D1/E1. | Cell Death & Disease

Fig. 3: The role of HMGA1 in the proportion of cells in different stages of the cell cycle and its effect on the expression of G1/S transition regulators cyclin D1/E1.

From: HMGA1 exacerbates tumor growth through regulating the cell cycle and accelerates migration/invasion via targeting miR-221/222 in cervical cancer

Fig. 3

a Representative cell cycle images of Siha at 48 h after transfection with empty plasmid or HMGA1 expression plasmid by flow cytometry. b, c, d Representative cell cycle images of Caski, MS751, and C33A at 48 h after transfection with 100 nmol siNC or 100 nmol specific siHMGA1 by flow cytometry. e The proportion statistics analysis of each cell cycle phase for a, b, c, and d. f The expression of cyclin D1 and cyclin E1 mRNA in Siha-HMGA1, MS751-siHMGA1, Caski-siHMGA1, C33A-siHMGA1, and their control cells by real-time PCR, the expression levels were normalized to GAPDH expression. g The proteins expression of cyclin D1 and cyclin E1 in Siha-HMGA1, MS751-siHMGA1, Caski-siHMGA1, C33A-siHMGA1, and their control cells by western blot analysis. siNC represents the siRNA negative control, and siHMGA1 represents the specific HMGA1 siRNA. *p < 0.05, **p < 0.01, and ***p < 0.001

Back to article page