Fig. 1: Glaucocalyxin A inhibited the cell viability and induced apoptosis in human osteosarcoma cells. | Cell Death & Disease

Fig. 1: Glaucocalyxin A inhibited the cell viability and induced apoptosis in human osteosarcoma cells.

From: Glaucocalyxin A exerts anticancer effect on osteosarcoma by inhibiting GLI1 nuclear translocation via regulating PI3K/Akt pathway

Fig. 1: Glaucocalyxin A inhibited the cell viability and induced apoptosis in human osteosarcoma cells.The alternative text for this image may have been generated using AI.

a The chemical structure of Glaucocalyxin A. b, c MTT and CCK8 assays were used to detect the cell viability of human osteosarcoma cells after different concentrations of Glaucocalyxin A for 24 and 48 h respectively. d, e MTT and CCK8 assays were used to detect the cell viability of human normal cells after different concentrations of Glaucocalyxin A for 24 and 48 h. f MG-63 cells were observed under an inverted light microscope after treatment with 2.5, 5 and 10 μM Glaucocalyxin A for 24 h (×200). g The morphology of nucleus in HOS and MG-63 cells was observed by DAPI staining (×400). h The apoptotic rates of HOS and MG-63 cells induced by Glaucocalyxin A were detected by Annexin V/PI double-staining assay. The results are shown as means ± SD from three independent experiments. **P < 0.01 compared with control group

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