Fig. 2: The influence of stable knockdown of ANXA5 on the cell growth of Hca-P cells.

a Western blotting assay indicated that ANXA5 was down-regulated by ~99% (P < 0.001) and ~53% (P < 0.001) in Hca-P-Anxa5-shRNA1 and -shRNA2 than the control group cells. Levels are normalized to α-Tubulin. Values mean ± S.D. of three independent experiments. b Compared with Hca-P-Anxa5-shControl, qRT-PCR assay showed Anxa5 levels decreased by ~91% (P = 0.0008) and ~17% (P = 0.0455) in Hca-P-Anxa5-shRNA1 and -shRNA2 cell lines. c The inhibition of ANXA5 knockdown on the colony forming capacity of Hca-P depending on its knockdown extent. Compared with Hca-P-Anxa5-shControl cells, the colony forming activity of Hca-P-Anxa5-shRNA1 decreased by 65.5% (P = 0.0003), while, no stastical difference was between Hca-P-Anxa5-shControl and -shRNA2. d MTT assay for the effect of ANXA5 knockdown on the proliferation of Hca-P cells. The proliferation decreases of Hca-P-Anxa5-shRNA1 compared with Hca-P-Anxa5-shControl were of stastical significations (P < 0.01). No proliferation differences were observed among Hca-P, Hca-P-Anxa5-shControl and -shRNA2. Values mean ± S.D. of three independent experiments